In one set of experiments, cells were grown to 70 to 80% confluence and then pretreated for 3 h with a glutaminase inhibitor (either the pan-GLS/GLS2 inhibitors 968 or SU1 or the GLS selective inhibitors BPTES or UP4), or with vehicle control, followed by infection of VeroE6 cells with SARS-CoV-2 (MOI 0.01, 2%FBS in DMEM) for 1 h at 37 °C, and HBECs or HCT8 cells with HCoV-OC43, (MOI 0.01, 2% FBS in RPMI medium) for 1 h at 33 °C. Here, GLS is linked to infection.