RYR2 and heart disorder: However, the Ca2+/CaM-dependent protein kinase II (CaMKII)-dependent phosphorylation of PLB at Thr 17 and RyR2 at Ser 2814 was increased in HFD hearts (Figure S3a,c,f), consistent with elevated CaMKII activities in cardiac diseases (M. E. Anderson et al., 2011).