To examine the role of Akt signaling at the time of reactivation, hESC-derived CD34+ HPCs were infected with TB40/E-GFP at a multiplicity of infection (MOI) of 2 for 48 hours, and viable, CD34+, GFP+ cells were sorted and seeded into long-term bone marrow culture (LTBMC) over stromal cell support to allow for the establishment of latent infection. Here, AKT1 is linked to disease arising from reactivation of latent virus.