The guideline group recommends that HCPs consider these points when diagnosing POI:Pregnancy should be excluded in women presenting with amenorrhea.Use of hormonal therapy (including oral, injectable, or long-acting contraceptives) may conceal or cause amenorrhea or irregular menstrual cycles, and potentially lower FSH concentrations. Some hormonal therapy (e.g. combined oral contraceptive) may need to be ceased before a diagnosis of POI can be confirmed.Women who had bilateral salpingo-oophorectomy (BSO) before age 40 have a diagnosis of POI, and additional diagnostic testing is unnecessary. Here, BRD2 is linked to amenorrhea.