Regarding age-associated immune alterations (Supplementary Fig. 4E and F), within the ALS group, we found no significant correlations between age and either the proportion of CD8+  GZMKhi TEMs (r = 0.5, P = 0.101) or the clonality of CD8+ T cells (r = 0.197, P = 0.562). This evidence concerns the gene CD8A and amyotrophic lateral sclerosis.