Specifically, these two cases exhibited notably high levels of PAX1 and JAM3 methylation (Patient 1: ΔCtPAX1 = 2.52, ΔCtJAM3 = 5.28, LBC: NILM; Patient 2: ΔCtPAX1 = 1.82, ΔCtJAM3 = 3.72, LBC: adenocarcinoma). This evidence concerns the gene PAX1 and adenocarcinoma.