Additionally, we attempted to characterize benign nevi, dysplastic nevi, non-invasive melanoma, and invasive melanoma in cdkn2b-/-/tp53-/- Xenopus tropicalis using a variety of commercial antibodies that target human melanoma markers (Zeb1, Gpnmb, Vim, Sox10, S100beta, Tyr, Mitf). The gene discussed is GPNMB; the disease is melanoma.