High-BCAA chow feeding did not alter food intake (Figure S4C,D), water intake (Figure S4E,F), fasting blood glucose levels (Figure S4G,H), and insulin resistance, as detected by glucose tolerance tests (Figure S4I,J) and insulin tolerance tests (Figure S4K,L) in both STZ/HFD and db/db mice, compared with normal chow-fed mice. This evidence concerns the gene INS and Insulin resistance.