Matrigel, a basement membrane matrix extracted from Engelbreth–Holm–Swarm mouse sarcoma containing ~60% laminin, 30% collagen IV, 8% nidogen-1/entactin, heparan sulfate proteoglycans, and a number of growth factors, such as transforming growth factor-beta 1, epidermal growth factor, and insulin-like growth factor was used as a positive control because of its beneficial effects during cell culture [20]. The gene discussed is NID1; the disease is sarcoma.