Given the increases in plasma total ghrelin and AG with dietary-induced weight loss [6, 11, 13-16, 18], which is also seen for total ghrelin in obesity and T2DM after DJBL insertion (though there was no dietary control group) [78], and the potential importance of the plasma AG/LEAP2 ratio in human eating behavior [17, 42], it will be important to measure plasma AG in this study. Here, GHRL is linked to obesity due to melanocortin 4 receptor deficiency.