LEAP2 and obesity due to melanocortin 4 receptor deficiency: In mice with normal weight, (i) a 40% caloric restriction over 2 weeks tended to decrease fasting plasma LEAP2 [31], while (ii) weight loss in diet-induced obesity (high-fat diet for 12 weeks) after return to normal chow for 4 weeks [17], (iii) a 50% caloric restriction over 14 days in mice eating normal chow [64], and (iv) a ketogenic diet with 75% dietary fat over 3 weeks leading to an approximately 14% weight loss in chow-fed mice [44], all decreased fasting plasma LEAP2.