induced chronic T cell receptor (TCR) signaling in vitro by stimulating T cells with anti-CD3 and anti-CD28 antibodies,32 and Trefny and colleagues repetitively stimulated NY-ESO-1 TCR-T cells using human leukocyte antigen-A2-positive T2 tumor cells loaded with NY-ESO-1 peptides.29 The gene discussed is CD28; the disease is neoplasm.