HTR1A and major depressive disorder: Notably, the same researchers conducted a pharmacological approach to show that WAY100135 (the 5-HT1A receptor antagonist) blocked the antidepressant-like effect of CBD on immobility time in rats.35 These results indicate that the antidepressant effects of CBD in a rat model of depression are associated with the downregulation of miR-16 and miR-135 in the vmPFC and are mediated by the 5-HT1A receptor.