LY6S and colorectal carcinoma: To further confirm whether the pro-CRC effect of F. nucleatum was LY6A dependent in vivo, we generated gut-specific Ly6a-KO mice by crossing Vil1CreERT2 mice with Ly6a-floxed (Ly6afl/fl) mice, resulting in VillinCreER2Ly6afl/fl offspring (Figure 3J and Supplemental Figure 6, C and D).