EDN1 and renal fibrosis: By detecting the levels of the fibrosis-related proteins SMA, ECA, TGF-β, and VIMENTIN, as well as the renal tubular injury–related factors AKLOTHO and EDN1, our results reveal that the extent of renal fibrosis and tubular injury in the WTDI/R mice was more severe than in the KODI/R mice (Figure 2).