We next employed a syngeneic orthotopic pancreatic cancer model where cells derived from KrasG12D, Trp53R172H, Pdx1-Cre (KPC) tumor bearing mice are injected into the pancreas of wildtype C57BL/6 mice, thereby recapitulating a human pancreatic cancer microenvironment (Matzke-Ogi et al., 2016). Here, PDX1 is linked to pancreatic neoplasm.