To derive more granularity on the potential roles of NK cell and T-cell populations in tumor control, tissue sections were stained using an optimized multiplex immunofluorescence panel and analyzed by HALO AI software to identify tumor cells (E-cadherin+ - blue), CD8+ T-cells (CD3+CD8+ - orange), CD4+ T-cells (CD3+CD8- - green), and NK cells (CD3-CD8-NK1.1+ - red) (Figure 4A, Figure 4—figure supplement 1A). Here, CD4 is linked to neoplasm.