From a selection of independently isolated KPC-derived cell lines, we first determined the cell line KPC-F as an appropriate model for human PDAC tumor based on the expression of epithelial (E-cadherin), mesenchymal (vimentin), and stromal (Collagen I, αSMA) markers as well as displaying growth kinetics (determined by MRI) amenable for the study and expression of high levels of CCL5 (Figure 2A, Figure 2—figure supplement 1A and B). The gene discussed is ACTA1; the disease is neoplasm.