Similarly, in the study by Zhang C and colleagues, the knockout of Sirt3 and the use of specific Sirt3 siRNA in mice with iothalamate-induced acute kidney injury and NRK-52E cells eliminated the renal protective effects of the medication, further confirming that activating Sirt3 in CI-AKI models, both in vitro and in vivo, can provide protection against oxidative stress, apoptosis, and inflammation, potentially improving CI-AKI in clinical practice (Zhang et al., 2021). Here, SIRT3 is linked to acute kidney injury.