To explore the roles of UGGT1 and UGGT2 in ERAD, UGGT1-KO, UGGT2-KO, and UGGT-DKO cell lines were generated from HCT116 diploid cells derived from human colonic carcinoma using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9. This evidence concerns the gene UGGT1 and colon carcinoma.