As illustrated in Figure 3A–D, Western blotting analysis revealed decreased expression levels of RXFP1, PI3K p55, and eNOS, as well as the reduced ratios of p‐AKT/AKT and p‐eNOS/eNOS in the DMED group, suggesting endothelial dysfunction in penis. This evidence concerns the gene RXFP1 and endothelial dysfunction.