NOS1 and epilepsy: To further distinguish the role of nNOS in glutamatergic DGCs and GABAergic interneurons in the development of epilepsy, we constructed Nos1 conditional knockout (nNOS cKO, Nos1loxp/loxp) mice, in which the exons 4–7 of Nos1 were flanked by two LoxP sites using the CRISPR/Cas9 system (Figs. 3D and EV3A).