To determine whether nNOS deficiency is involved in pilocarpine-induced epilepsy in mouse models, we measured hippocampal nNOS expression by RT-qPCR and western blot in the DG of the hippocampus at different time points after pilocarpine-induced SE (275 mg/kg, s.c., 1 time) (Fig. 1G–J). Here, NOS1 is linked to epilepsy.