CBD vs. control:↓ viability of NSCLC cell and spheres with different sensibility.↓ sphere formation.↓ expression levels of: SOX2, Oct‐4 and CD133 (in A549 spheres); SOX2 and CD133 (in H1299 spheres) and SOX2, CD44, CD133 and Oct‐4 (in H69 spheres).↑ annexin‐positive cells in A549 spheres (~30%), in H1299 spheres (10%–15%) and in H69 spheres.↑ of caspase 3/7 in A549, H1299 and H69 spheres.↑ expression of TP53 (3‐fold), CDKN1A (5‐fold) and pro‐apoptotic genes BAK1, BAX, and BAD (~1.8‐fold) in A549. This evidence concerns the gene SOX2 and non-small cell lung carcinoma.