Recombinant LcrV-HSP70, designed for Yersinia pestis and M. tuberculosis, induced significant expression of IFN-γ and TNF-α in immune mice, providing 100% protection against plague and leading to a marked decrease in the colony-forming units (CFU/mL) of Yersinia and Yersinia pseudotuberculosis in blood and spleen [57]. This evidence concerns the gene TNF and plague.