Although it is unclear whether αζDKO Tfr-cells were impaired in suppressive function, they gained expression of Tfh-associated cytokine Il21 as well as Th2-associated cytokines Il4 and Il13, which might have contributed to the IgG1/IgE-predominant antibodies and autoimmunity in Treg-αζDKO mice. This evidence concerns the gene IGHE and Autoimmunity.