We chose to ablate a single Traf2 allele to avoid the confounding effects of cardiomyopathy with ablation of both alleles as we and others have described.27, 45 Loss of one Traf2 allele is well tolerated in the mouse myocardium.45 Inducible adult-onset ablation of one Traf2 allele in R120G TG mice from 8 weeks of age (Fig. 6A) resulted in worsening of left ventricular systolic function (Fig. 6B, C) at 20 weeks of age without affecting left ventricular end-diastolic dimension (Supplementary Figure S6A). Here, TRAF2 is linked to cardiomyopathy.