Li et al., utilizing neuroblastoma cells with ULK1 knockout, discovered that the formation of PHEV-induced autophagosomes does not necessitate the participation of ULK1, indicating that PHEV-induced autophagy represents a non-canonical process, independent of the AMPK-mTORC1-ULK1 signaling pathway (Li Z. et al., 2021). The gene discussed is ULK1; the disease is neuroblastoma.