To examine the impact of HFD on neuronal cell death and necroptosis after ischemic stroke, we double labeled NeuN (neuronal specific marker) with TUNEL (labeling truncated DNA representing cell death) and phosphorylated RIPK3 (pRIPK3, an activated kinase promoting necroptosis), and found that both percentages of TUNEL+ NeuN+ and pRIPK3+ NeuN+ neuron were significantly elevated in the peri‐infarct region of HFD‐treated mice compared to that in ND‐treated mice 3 days after MCAO (Figure 1D,E). This evidence concerns the gene RBFOX3 and ischemic stroke.