Our observations revealed that anti-Ly6G administration markedly suppressed NETs formation within non-cryoablation lesions, significantly reducing tumor volume in the cryoablation + anti-PD1 + anti-Ly6G group compared to the cryoablation + anti-PD1 group (Fig. 5 L, M, Supplementary Figure S5E, F, Supplementary Figure S7C, D). Here, PDCD1 is linked to neoplasm.