To further confirm the effects of NTD and CTD of Hsp90α on FASN related lipid accumulation, we transfected HCC cells with plasmids with full length of Hsp90α (HA-tagged full-length), Hsp90α NTD deletion (HA-tagged MD-CTD) or CTD deletion (HA-tagged NBD-MD) (Fig. 4J). Here, HSP90AA1 is linked to hepatocellular carcinoma.