An AAV viral construct with shRNA against Trpc6 (AAV9-U6-shRNA(Trpc6)-CMV-GFP) was injected into the VTA of mice, to knockdown TRPC6; efficiency of this knockdown was assessed by using qPCR measuring the mRNA level of Trpc6 in the VTA tissue (Figure 5C), and efficiency of viral infection into the VTA DA neurons (marked by DAT) was observed through visualization of GFP (Figure 5D). This evidence concerns the gene TRPC6 and viral infectious disease.