Importantly, male mice with CRS-induced depression also showed a significant downregulation of TRPC6 protein in the VTA (0.38±0.06 fold, N=10 for control and N=14 for CRS, Two-sample t-test, t=7.369, df = 22, 95% CI: –0.8006 to –0.4489, p<0.0001, Figure 6—figure supplement 4J). Here, TRPC6 is linked to major depressive disorder.