To begin exploring the mechanisms by which FTO might regulate cell cycle progression in AML cells, we performed cell cycle analysis of THP-1 and MV411 cells by using flow cytometry, cell cycle analysis revealed that silencing FTO led to a decreased percentage of THP-1 and MV411 cells in G0-G1 phase and an increased percentage of cells in S phase compared to the control group (Fig. 7c and d). This evidence concerns the gene FTO and acute myeloid leukemia.