To externally assess our LASSO model’s ability to identify malignant cells, we applied it to another RCC single-cell RNA-Seq (scRNA-seq) dataset from Li et al. [17] All genes in the LASSO model were present except for WIF1, which is due to low coverage in the scRNA-seq because it is a whole transcriptome platform. Here, WIF1 is linked to renal cell carcinoma.