Data from qPCR assays showed that knockdown of KDM1A significantly increased the expression of the proinflammatory genes TNF, IL-1B, IL-6, and NR4A1 in TE1 (Fig. 3H, p = 0.005 for TNF, and p < 0.0001 for IL-6, IL-1B, NR4A1, respectively) and K410 cells (Fig. 3I, p = 0.002 for TNF, p = 0.0002 for IL-1B, p = 0.035 for IL-6, and p = 0.002 for NR4A1, respectively), implicating that KDM1A may remodel the TME by inhibiting the expression of TNFα-NF-κB-dependent inflammatory genes, especially the TNF, IL-6, IL-1B, and NR4A1 genes, in ESCC. This evidence concerns the gene TNF and esophageal squamous cell carcinoma.