Taking advantage of a novel in situ DSP tool [26], we comprehensively characterized the spatial distribution of multiple immune proteins in ESCC and lymphocytes from the TME in KDM1A high and low tissues, respectively, using the cell markers PanCK indicative of ESCC (PANCK+) and CD45 of lymphocytes (CD45 + ). Here, PTPRC is linked to esophageal squamous cell carcinoma.