After 30 hours of seeding, we observed high levels of phosphorylated EGFR (pEGFR) in the two BRAF class 3–mutated cell lines (PDX-ADK-36 and ADK-14) and in the two EGFR-mutated cell lines (HCC-827 and PC-9), but not in the KRAS-mutated (ADK-17), BRAF class 1–mutated (HCC-364), and BRAF class 2–mutated (NCI-H1395) cell lines (Fig 3A). Here, BRAF is linked to hepatocellular carcinoma.