The analysis results demonstrated that the prognostic gene SPP1 was significantly positively correlated with 14 immune cell subsets in the liver hepatocellular carcinoma (LIHC), including B cells, cancer-associated fibroblasts, macrophages, macrophages (M0, M1, M2), monocytes, neutrophils, myeloid dendritic cells, macrophages/monocytes, activated NK cells, CD4+Th2 T cells, regulatory T cells (Tregs), and myeloid-derived suppressor cells (MDSCs) (Figure 7G). The gene discussed is CD4; the disease is cancer.