In our exploration of the network mechanism by which ML exerts its metabolic regulatory effects on the livers of rats with type 2 diabetes (T2D), we have discerned that ML significantly enhances the expression of key enzymes such as ACSL5 and DLAT, this upregulation facilitates the β-oxidation of fatty acids in the liver, a critical process for energy metabolism in T2D rats. This evidence concerns the gene ACSL5 and type 2 diabetes mellitus.