On the other hand, we found that after TW80-SeNPs was absorbed by MG63 cells and internalized into cells, it was gradually metabolized into SeIV, which regulated various selenoproteins in cancer cells, reduced the expression levels of SeIH, SeIM, SeIO, and SeIP, and increased the expression levels of Gpx4, TR1, and Sep15. This evidence concerns the gene GPX4 and cancer.