The in vivo acute and chronic toxicity analysis and biodistribution determinedthrough micropositron emission tomography (μPET)/microcomputedtomography (μCT) imaging showed the safety of this formulation.Blood glucose dynamics upon administration of glucagon-micelle inhealthy mice demonstrated successful regulation of glucose levelsby reverting and preventing insulin-induced hypoglycemia. The gene discussed is INS; the disease is Hypoglycemia.