To get further insight into the role of YAP1 in the TME and to understand the influence of YAP1 expression on the infiltration of MDSCs and macrophages, MDSCs were isolated from tumor mass developed from MB49 YAP1-Sh and Sh-control clones and primary macrophages were isolated from the intraperitoneal cavity of WT mice. The gene discussed is YAP1; the disease is neoplasm.