For further validation, we performed immunohistochemical (IHC) staining of Gr-1 and CD8 using the tumor tissue derived from YAP1-expressing and YAP1-downregulated MB49 cells, and our findings were consistent with flow cytometric analysis (Supplemental Figure 2G): decreased numbers of MDSCs (Gr-1) and increased numbers of CD8+ T cells in YAP1-Sh tumors. The gene discussed is YAP1; the disease is neoplasm.