In addition, immunohistochemical analysis demonstrated that proliferative and migratory biomarkers Ki67, PCNA, MMP9, N‐cad, Vimentin, and EZH2 were reduced, while E‐cad expression was elevated in tumor tissues from lnc191‐knockdown xenograft mice, and the expression of p‐ERK was also reduced, suggesting that knockdown of lnc191 suppressed ESCC proliferation, metastasis and p‐ERK pathway in vivo (Figure 6I; Figure S4A, Supporting Information). This evidence concerns the gene MMP9 and esophageal squamous cell carcinoma.