Our results revealed that tumor cell proliferation and invasion were significantly reduced, and apoptosis was enhanced in the FAM3C‐overexpressing group compared to the control‐overexpressing group,alongside elevated E‐cadherin levels both in in vivo and in vitro experiments and decreased expression of Snail, Twist, HES1, HES5, and HEY1. This evidence concerns the gene HES5 and neoplasm.