After strict quality control, a total of 26185 cell transcriptomes were retained for subsequent analysis, and twelve major cell clusters with the expression of known marker genes were identified (Figure 1A, Figure S1A, Table S1), including MDMs, Microglia (MGs), Dendritic cells (DCs), Natural Killer cells (NK cells), Neutrophils, CD4+ T cells, Regulatory T cells, CD8+ T cells and four malignant clusters (MES, AC, OPC and NPC), consistent with previous findings that malignant cells exhibited strong intertumoral heterogeneity 3. Here, CD8A is linked to nasopharyngeal carcinoma.