To investigate the role of SPHK1 in HNSCC cells, we analyzed a public single-cell dataset (GSE172577; www.ncbi.nlm.nih.gov/geo) comprising primary tumor tissues from six untreated patients with oral squamous cell carcinoma (39,064 cells; Figure 1A). The gene discussed is SPHK1; the disease is head and neck squamous cell carcinoma.