We measured IFN-γ concentrations in the tumor lysates by enzyme-linked immunosorbent assay (ELISA) and found that the IFN-γ level in the whole tumor tissue reached its peak at Day 1.5 after T cell transfer, reaching a concentration of 0.25 pg/mg of tissue and decreasing thereafter (Figure 3A). The gene discussed is IFNG; the disease is neoplasm.