In order to purify and sequence mRNAs that are translated in motor neurons during the progression of ALS, the Ubqln2P497S mutation was crossed into a mouse line containing both RiboTag (Ribo) (24) and Chat-Cre to drive the expression of HA-tagged RPL22, and thus HA-tagged ribosomes, in a motor neuron-specific manner, as we have done previously (23). This evidence concerns the gene RPL22 and amyotrophic lateral sclerosis.