BRAF and colorectal carcinoma: To model the response of BRAF-V600E-mutant CRC to both targeted therapy and chemotherapy, we used a Smad4f/f; BrafV600E/+ mouse model, which emulated the patient's mutational profile.10-12 Organoids were prepared from wildtype intestinal crypts and from tumors found in Smad4f/f; BrafV600E/+ mice (n = 3).