While we were unable to establish a link between some of the risk factors for PPROM classically described in the literature and mechanical fragility of the membranes, biochemical analysis (collagen, elastin) by multiphoton microscopy of fetal membranes according to PPROM risk factors could help us to understand the pathogenesis of these elements, and would be an interesting complement to our study of the mechanical behavior of these membranes. Here, ELN is linked to preterm premature rupture of the membranes.