To determine differences in localization between DENV2-NS2B3 protease when expressed alone versus in the context of the replicating virus, we generated two additional reporters; one with EGFP fused to sec61β (hereafter EGFPβ), and the other with EGFP fused to the transferrin transmembrane (TM) domain followed by a KDEL ER retention signal (hereafter EGFPTM), the latter of which was used previously in a flavivirus infection reporter system [39]. This evidence concerns the gene TF and Flavivirus Infections.