We provide a comprehensive analysis of the histologic, morphometric, and immunohistochemical features of the cornea in this mouse model with heterozygous Pax6+/– genotype, relative to wild-type (WT; Pax6+/+) littermates, showing that the model mimics the human disease and provides a previously inaccessible potential therapeutic window for AAK, to facilitate detailed mechanistic and therapeutic investigations of relevance for human congenital aniridia. The gene discussed is PAX6; the disease is isolated aniridia.