Besides, the toxicity of mutant SOD1 also damages the removal of abnormal aggregates by autophagy, which further produces the excessive aggregation and deposition of pathological proteins including the mutated SOD1 to generate the insoluble SOD1 ubiquitin-positive inclusion body, creating a vicious cycle that leads to cell death in the pathogenesis of ALS. This evidence concerns the gene SOD1 and amyotrophic lateral sclerosis.