As depicted in Fig. 1A, approximately nine out of nineteen cuproptosis-related genes, including NFE2L2, NLRP3, ATP7A, SLC31A1, LIPT2, MTF1, CDKN2A, GCSH and DLST, displayed significant differences between normal and bladder cancer tissues in the TCGA database. This evidence concerns the gene NLRP3 and urinary bladder cancer.