Furthermore, we confirmed the downregulation of TP63 and KRT5, as well as the upregulation of TEAD4 and KRT8/18 during HNSCC metastasis, upon analyzing two previously published scRNA-seq data sets from primary and metastatic HNSCC (Supplemental Fig. S4B–J; Puram et al. 2017; Sharma et al. 2018). This evidence concerns the gene KRT5 and head and neck squamous cell carcinoma.